NEON Biorepository Benthic Macroinvertebrate Collection (Fluid Preserved Chironomids)

Chironomid subsample from the taxonomy laboratory (NEON sample class: inv_persample_in.chironomidVialID). Benthic macroinvertebrate samples are collected three times per year at wadeable stream, river, and lake sites during aquatic biology bout windows, roughly in spring, summer, and fall. Samples are collected using the most appropriate sampler for the habitat type, including Surber, Hess, hand corer, modified kicknet, D-frame sweep, and petite ponar samplers. Macroinvertebrates are sampled using a percent-based macrohabitat approach. In wadeable streams, samples are collected in the two most dominant habitat types (e.g. riffles, runs, pools, step pools) within each 1 km-long wadeable stream site. In lakes, samples are collected near the buoy, inlet, and outlet sensors using a petite ponar, and in littoral areas using a D-frame sweep. In rivers, samples are collected near the buoy and two other deep-water locations using a petite ponar sampler, and in littoral areas using a D-frame sweep or large-woody debris sampler. Field protocols differ depending on the habitat and substrate being sampled, but all samples are collected from the surface of the natural substratum in each habitat. Samples are preserved in ethanol in the field, returned to the domain support facility for a preservative change.  A small volume of glycerol was added to samples processed prior to October 2021. Samples are shipped to a taxonomy lab for sorting and identification, including count of each taxon per size class (to nearest mm) and identification to lowest practical taxon (genus or species). Chironomid subsamples are preserved in 70-95% ethanol and archived in a temperature (17-18°C) and humidity controlled environment. See related links below for protocols and NEON related data products.

摇蚊（Chironomid）分类学实验室子样本（NEON样本类别：inv_persample_in.chironomidVialID）。底栖大型无脊椎动物（Benthic macroinvertebrate）样本每年在可涉水溪流、河流及湖泊站点采集三次，采集时间处于水生生物学采样窗口期内，大致为春季、夏季和秋季。样本采集采用针对栖息地类型的最适采样器，包括Surber采样器、Hess采样器、手动取芯器、改良踢网、D型网扫样器及小型波纳尔采样器。大型无脊椎动物采用基于百分比的宏观生境法采样。在可涉水溪流中，样本采集于每个1公里长溪流站点内的两种优势栖息地类型（如急流、缓流、深潭、阶梯潭）。在湖泊中，样本通过小型波纳尔采样器采集于浮标、入口及出口传感器附近，通过D型网扫样器采集于沿岸区域。在河流中，样本通过小型波纳尔采样器采集于浮标及另外两处深水位置，通过D型网扫样器或大型木质残体采样器采集于沿岸区域。野外规程因采样的栖息地和基质而异，但所有样本均采集自各栖息地的天然基质表面。样本在野外以乙醇固定，返回区域支持设施后更换防腐剂。2021年10月前处理的样本添加了少量甘油（Glycerol）。样本被运送至分类学实验室进行分拣与鉴定，包括各体型等级（精确至毫米）下每个分类单元（taxon）的计数，以及鉴定至最细实用分类单元（属或种）。摇蚊子样本以70-95%乙醇保存，并存档于温湿度控制环境（温度17-18°C）。有关规程及NEON相关数据产品，请参见下方相关链接。