Florescent in situ hybridization with rDNA probes to the chromosomes of annual Brachypodium species.

The aim of the study was to draw a complex picture of rRNA gene evolutionary trajectories in two Brachypodium hybridum lineages of distinct evolutionary ages and cross directions. We aimed to address the following questions: (i) are the evolutionary trajectories of 35S and 5S rDNA homoeologues similar or not?; (ii) is there a relationship between the direction of the cross and rDNA homoeologues loss in B. hybridum?; (iii) do the rRNA genes in the studied B. hybridum lineages of different evolutionary ages follow the same evolutionary pathways?Description of the database: Microphotographs (TIF files) of mitotic metaphase chromosomes after fluorescence in situ hybridisation (FISH) with 25S rDNA and 5S rDNA as probes. All microphotographs were acquired using a Zeiss AxioImager.Z.2 fluorescent microscope equipped with an AxioCamHMr camera (Zeiss, Oberkochen, Germany). The microphotographs were processed uniformly using ZEN 2.3 Pro (Zeiss). Number of TIF files: 12.

本研究旨在描绘两个进化年代及杂交方向各异的布氏偃麦草（Brachypodium hybridum）谱系中核糖体RNA（rRNA）基因的复杂进化轨迹。我们试图解答以下问题：(i) 35S与5S核糖体DNA（rDNA）同源基因的进化轨迹是否相似？(ii) 杂交方向与布氏偃麦草中rDNA同源基因的丢失是否存在关联？(iii) 不同进化年代的布氏偃麦草研究谱系中，rRNA基因是否遵循相同的进化路径？
数据库描述：使用25S rDNA和5S rDNA作为探针进行荧光原位杂交（FISH）后获得的有丝分裂中期染色体显微照片（TIF文件）。所有显微照片均通过配备AxioCamHMr相机的蔡司AxioImager.Z.2荧光显微镜（德国奥伯科亨市蔡司公司）采集。显微照片通过ZEN 2.3 Pro软件（蔡司公司）进行统一处理。TIF文件数量：12。