Longitudinally stable T cell and innate immune activity distinguish healthy adult immunotypes I

Using cytometric, proteomic, and transcriptomic longitudinal data derived from whole blood, we identified four robust longitudinally stable immunotypes associated with innate immune activation and T-cell function in healthy adults. We enrolled and comprehensively phenotyped a cohort of 100 healthy adults aged 25-35 and 55-65 years who were longitudinally followed for 10 visits over 2 years. Using mass cytometry features that remain stable within - but differ between – individuals, we identified four stable immunotypes. We characterized these immunotypes using whole blood RNA sequencing, Olink proteomic profiling, and whole blood ex vivo stimulation.