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Topo mutants psoralen photobinding

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE16758
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We present a DNA array-based method for the genome-wide analysis of Psoralen photobinding in Saccharomyces cerevisiae cells. PB was found reduced in subtelomeric regions and increased in the subset of genes with smallest nucleosomal density. The analysis includes 3 repeats of the wild type strain (JCW25) at 30ºC and 3 more at 37ºC, 3 repeats of the double top1 (delta) top2ts mutant at 37ºC and 3 repeats of naked genomic DNA treated with trimethyl psoralen (TMP) in the same way to be used as a control of unspecific binding.
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2012-03-21
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