Carbohydrates on the growth of Cattleya schilleriana Reichb.f. seedlings (Orchidaceae)

ABSTRACT: The aim of the present study was to evaluate the effects of carbohydrate supplementation on the propagation of the orchid Cattleya schilleriana. The 120-d-old seedlings were subcultured in fructose-, glucose-, or sucrose-supplemented (0, 15, 30, and 45g L-1) ½ MS culture medium (half-strength macronutrient concentrations), using a completely random design with four repetitions per treatment. After 120d of treatment, root number and length, leaf number and length, and fresh weight were evaluated, and seedling survival was evaluated after 75d of acclimatization in a greenhouse. The in vitro growth data were submitted to regression analysis, whereas the percentage survival data were analyzed using ANOVA and Tukey’s test. Both in vitro growth and ex vitro survival were lowest when the plantlets were grown in the absence of a carbohydrate source and highest (>90% survival) when supplemented with glucose. According to our findings, the addition of either glucose (30g L-1) or sucrose (30g L-1) is recommended for mass propagation of C. schilleriana.

摘要：本研究旨在评估碳水化合物补充剂对卡特兰（Cattleya schilleriana）繁殖的影响。将培养120天的幼苗继代培养于添加果糖、葡萄糖或蔗糖（浓度分别为0、15、30和45g·L⁻¹）的1/2 MS培养基（半强度大量元素浓度）中，采用完全随机设计，每个处理设置4次重复。处理120天后，统计幼苗的根数、根长、叶数、叶长及鲜重；将幼苗置于温室驯化75天后，评估其成活率。体外培养生长数据采用回归分析进行处理，成活率数据则采用方差分析（ANOVA）与图基（Tukey）检验进行分析。当培养基中未添加碳水化合物时，幼苗的体外生长与离体成活率均最低；而添加葡萄糖时，各项指标均达到最高（成活率＞90%）。基于本研究结果，建议在卡特兰规模化繁殖中添加30g·L⁻¹的葡萄糖或蔗糖。