CIL:9061, Nephrotoma suturalis, spermatocyte. In Cell Image Library

The events of meiosis I and II in a living insect spermatocyte beginning with the breakdown of the nuclear envelope at diakinesis through to the completion of cytokinesis. Testes from the Crane fly Nephrotoma suturalis were observed with time-lapse polarization using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 1.5x zoom lens. Image acquisition and processing Camera: Images captured every 2 min over 5 hr 18 min by a QImaging Retiga EXi CCD camera. A movie prepared from this stack of images is included in the grouped set

本数据集完整记录了活体昆虫精母细胞内减数分裂I期与II期的动态过程，时间跨度覆盖终变期（diakinesis）核膜崩解至胞质分裂（cytokinesis）完成的全部阶段。实验所用样本为大蚊（Crane fly）Nephrotoma suturalis的睾丸组织，采用搭载液晶偏振光显微镜（liquid crystal polarized light microscopy, LC-PolScope, CRi, 美国马萨诸塞州沃本市）系统的尼康Microphot SA显微镜开展延时偏振成像实验，配置参数包含60x/1.4 PlanApo油浸物镜、1.4数值孔径（NA）油浸聚光镜以及1.5x变焦镜头。图像采集与处理环节：使用QImaging Retiga EXi型CCD相机，每2分钟采集一张图像，总拍摄时长为5小时18分钟。由该图像序列堆栈生成的视频影片已纳入本组数据集。