A specific IL6 polymorphic genotype modulates the risk of T. cruzi parasitemia while IL18, IL17A and IL1B variant profiles and HIV infection protect against cardiomyopathy in Chagas disease

Background: Chagas disease caused by Trypanosoma cruzi (T. cruzi) affects approximately six million individuals worldwide. Clinical manifestations are expected to occur due to the parasite persistence and host immune response. Herein we investigated potential associations between IL1B, IL6, IL17A or IL18 polymorphism profiles and cardiomyopathy or T. cruzi parasitemia, as well as the impact of HIV infection on cardiopathy.

Methods: 206 patients and 90 control individuals were analyzed. IL1B rs1143627 T>C, IL6 rs1800795 C>G, IL17A rs2275913 G>A, IL18 rs187238 C>G, and IL18 rs1946518 C>A SNVs were analyzed by real-time PCR and T. cruzi parasitemia by PCR.

Results: Our data revealed association between a cytokine gene polymorphism and parasitemia never previously reported. The IL6 rs1800795 CG genotype lowered the risk of positive parasitemia (OR=0.45, 95% CI 0.24–0.86, P=0.015). Original findings included associations between IL17A rs2275913 AA and IL18 s1946518 AA ge...

背景：由克氏锥虫（Trypanosoma cruzi，简称T. cruzi）引起的查加斯病（Chagas disease）全球影响约600万人。临床症状的出现与寄生虫持续存在及宿主免疫反应相关。本研究旨在探讨IL1B、IL6、IL17A或IL18基因多态性谱与心肌病或T. cruzi寄生虫血症之间的潜在关联，以及HIV感染对心脏病的影响。

方法：对206例患者和90名对照个体进行分析。采用实时聚合酶链式反应（real-time PCR）检测IL1B rs1143627 T>C、IL6 rs1800795 C>G、IL17A rs2275913 G>A、IL18 rs187238 C>G及IL18 rs1946518 C>A单核苷酸变异（SNVs），并通过聚合酶链式反应（PCR）检测T. cruzi寄生虫血症。

结果：本研究数据揭示了此前未报道过的细胞因子基因多态性与寄生虫血症之间的关联。IL6 rs1800795 CG基因型降低了阳性寄生虫血症的风险（比值比（OR）=0.45，95%置信区间（95% CI）0.24–0.86，P值（P）=0.015）。原创性发现包括IL17A rs2275913 AA基因型与IL18 s1946518 AA基因...