Additional file 1 of Combined analysis of the metabolome and transcriptome provides insight into seed oil accumulation in soybean

Additional file 1: Figure S1. The oil content of the 30 soybean varieties. Student’s t-test were carried the significance levels (*P<0.05, **P<0.01). Figure S2. The content of flavonoid-related metabolites in the three comparison groups. Red represents up-regulated, and blue represents down-regulated. Figure S3. KEGG enrichment analysis p-value histogram of the differentially expressed genes (DEGs) and differentially abundant metabolites (DAMs) of the three comparison groups. A. FHO vs. FLO, B. THO vs. TLO, C. HO vs. LO. Blue represents gene, and green represents metabolite. Figure S4. A. Total number of DEG TFs in the three comparison groups. B. Number of various DEG TFs in the three comparison groups. Figure S5. Expression levels of 10 candidate genes in DN47 and DN50 soybean germplasms at the R6 growth period. Purple column represents DN50, blue column represents DN47. Student’s t-test were carried the significance levels (*P<0.05, **P<0.01). Figure S6. Correlations of the expression levels of the qRT-PCR and transcriptome data in the three comparison groups. A. FHO vs. FLO, B. THO vs. TLO, C. HO vs. LO. Table S1. Oil content of the 30 soybean varieties. Table S2. Statistics of differential genes related to oil synthesis. Table S3. Classification of metabolites related to lipid synthesis. Table S4. Co-expression analysis of lipid-related metabolites and genes. Table S5. Co-expression analysis of transcription factor and lipid-related metabolites. Table S6. Primers used for qRT-PCR.

附加文件1：图S1。30个大豆品种的含油量。采用学生t检验（Student’s t-test）进行显著性检验，显著性水平为*P<0.05，**P<0.01。图S2。三个比较组的类黄酮相关代谢物含量。红色代表上调，蓝色代表下调。图S3。三个比较组的差异表达基因（differentially expressed genes, DEGs）与差异丰度代谢物（differentially abundant metabolites, DAMs）的KEGG富集分析p值柱状图。A. FHO vs. FLO，B. THO vs. TLO，C. HO vs. LO。蓝色代表基因，绿色代表代谢物。图S4。A. 三个比较组的差异表达基因转录因子总数量；B. 三个比较组中各类差异表达基因转录因子的数量。图S5。10个候选基因在R6生育期的DN47与DN50大豆种质中的表达水平。紫色柱代表DN50，蓝色柱代表DN47。采用学生t检验进行显著性检验，显著性水平为*P<0.05，**P<0.01。图S6。三个比较组中实时荧光定量PCR（qRT-PCR）与转录组数据的表达量相关性。A. FHO vs. FLO，B. THO vs. TLO，C. HO vs. LO。表S1。30个大豆品种的含油量。表S2。油脂合成相关差异基因统计。表S3。脂质合成相关代谢物分类。表S4。脂质相关代谢物与基因的共表达分析。表S5。转录因子与脂质相关代谢物的共表达分析。表S6。qRT-PCR所用引物。