Confocal microscopy image stacks from "Temporal integration of auxin information for the regulation of patterning"

This dataset contains raw images in CZI format (Zeiss) of shoot apical meristems (SAM) from <em>Arabidopsis thaliana </em>transgenic lines <strong>qDII-pCLV3-pDR5</strong> or <strong>qDII-pCLV3-PIN1</strong>. See <em>(Galvan-Ampudia and Cerutti et al.) </em>for detailed information. This data constitutes the input of the <strong>sam_spaghetti</strong> pipeline (https://gitlab.inria.fr/mosaic/publications/sam_spaghetti) and can be processed using the scripts and examples provided in the package. <strong>File information:</strong> File names containing qDII-CLV3-DR5 have the following data: Channel 1: <em>DII-VENUS-N7</em> Channel 2: <em>pDR5:2xmTurquoise2</em> Channel 3: <em>pRPS5a:TagBFP-SV40</em> Channel 4: <em>pCLV3:mCherry-N7</em> File names containing qDII-CLV3-PIN1-PI have the following data: Channel 1: <em>DII-VENUS-N7</em> Channel 2: <em>pPIN1:PIN1-GFP</em> Channel 3: <em>Propidium Iodide (cell walls)</em> Channel 4: <em>pRPS5a:TagBFP-SV40</em> Channel 5: <em>pCLV3:mCherry-N7</em> Time-lapse sequences are identified as follows: <strong>qDII-CLV3-DR5-E27-LD-SAM7.czi</strong> <strong>qDII-CLV3-DR5-E27-LD-SAM7-T5.czi</strong> <strong>qDII-CLV3-DR5-E27-LD-SAM7-T10.czi</strong> where: <strong>qDII-CLV3-DR5</strong> indicates the line <strong>E$$-LD</strong> (e.g. E25-LD, E27-LD, etc) indicates independent biological replicas <strong>SAM$</strong> is the meristem (technical replica) <strong>T$</strong> indicates the time elapsed after the first image (in hours) For example <strong>qDII-CLV3-DR5-E27-LD-SAM7-T5.czi</strong> is an image of the 7th SAM of the set E27, acquired 5 hours after the first image.