Length and weight of F0 wild-caught adults Gasterosteus aculeatus (males and females) used for breeding in heatwave experiment

Fish were brought to the laboratory at the AWI Wadden Sea Station Sylt and used as breeding adults for a multi-generation laboratory experiment investigating the effects of marine heatwaves on stickleback fitness-related traits. These F0 generation wild-caught adults (males and females) were acclimated to three heatwave scenarios (no heatwave control, single heatwave or double heatwave) for three months before breeding (starting 12 June 2022) via artificial fertilization.

鱼类被带到阿尔弗雷德·韦格纳研究所（AWI）叙尔特岛瓦登海站的实验室，作为繁殖成体用于一项多代实验室实验，该实验旨在研究海洋热浪对棘鱼适合度相关性状的影响。这些F0代野生捕获的成体（雄性和雌性）在通过人工授精进行繁殖（始于2022年6月12日）前，已适应三种热浪情景（无热浪对照、单次热浪或两次热浪）达三个月。