Dyadic encounter unfamiliar female pairs over 2 days – 16p11.2 Del mouse strain – Del:+-Del/+ - 5194_5231

We evaluated the social interactions and communication between unfamiliar individuals in pairs. For these recordings of social behaviour and ultrasonic communication, we focused on pairs of individuals since we currently cannot identify the emitter of USVs when animals were interacting closely. Therefore, we recorded undisturbed dyadic interactions between two unfamiliar individuals (from two different housing cages) of the same age (14-20 weeks of age) and genotype for 47h (two days and nights). For that purpose, we coupled the Live Mouse Tracker system (plugin 931) with one CM16/CMPA microphone (Avisoft Bioacoustics, Glienecke, Germany) connected to the Avisoft Ultrasound Gate 416 (300 kHz sampling rate, 16-bit format; trigger: level of this channel; pre-trigger: 1 s; hold time: 1 s; duration > 0.005 s; trigger event: 2 % energy in 25-125 kHz with entropy < 50%; Avisoft Bioacoustics, Glienecke, Germany). LMT and Avisoft systems were synchronised based on the protocol described in (de Chaumont et al., 2021). Each upload includes the sqlite database from LMT (processed, with USV synchronised) and a zip file with all USVs sequences automatically recorded by Avisoft.

本研究评估了成对陌生个体间的社交互动与交流行为。针对此类社交行为与超声通讯的录制工作，我们聚焦于成对个体，原因在于当动物近距离互动时，目前无法识别超声发声（USVs）的发出者。因此，我们对来自不同饲养笼的同年龄（14~20周龄）、同基因型的两只陌生个体间的无干扰成对互动开展了为期47小时（两天两夜）的录制。为此，我们将活体小鼠追踪系统（Live Mouse Tracker，插件931）与一台CM16/CMPA型麦克风（德国格利尼克Avisoft Bioacoustics公司出品）相连，该麦克风接入Avisoft Ultrasound Gate 416设备，其参数设置为：采样率300 kHz，16位格式；触发条件：该通道电平；预触发时长1秒；保持时长1秒；有效时长>0.005秒；触发事件：25~125 kHz频段内能量占比2%且熵值<50%，该设备同样由德国格利尼克Avisoft Bioacoustics公司出品。活体小鼠追踪系统（LMT）与Avisoft系统依据de Chaumont等人2021年发表的实验方案完成同步校准。每份上传文件均包含来自LMT的SQLite数据库（已完成处理并完成USVs同步），以及一个包含Avisoft自动录制的全部USVs序列的压缩包。