Next Generation Sequencing Facilitates Quantitative Analysis of CRC cells with low DUOX2 expression compared with the control group

Purpose: The purpose of this study was to analyze changes in the cell genome along with the downregulation of DUOX2, as well as changes in gene enrichment pathways, so as to find the downstream target genes of DUOX2 Conclusions: Our study is the first detailed analysis of DUOX2's effect on the genome of CRC cells.We concluded that changes in DUOX2 gene levels can lead to activation of multiple tumor-related signaling pathways, and that NGS provides a more comprehensive and accurate method for quantitative and qualitative assessment of cell mRNA content. Overall design: CRC cells with low DUOX2 expression compared with the control group