A longitudinal model of human neuronal differentiation for functional studies of schizophrenia polygenic risk

Human neural stem cells were differentiation to a neuronal lineage over 30 days. Genome-wide gene expression was collected at seven time points in at least triplicates. We used these expression profiles to identify longitunal gene clusters that are expressed over time and map GWAS disease risk onto this. We show that schizophrenia disease is enriched in specific clusters across in vitro neuronal differentiation, which could make this an applicable model to study this disease. A longitudinal experimental design with seven time points and at least three replicates per time point. All samples come from the same genetic background (H9/WA09 NSC)