ECLIPSE. WP1 Task 1.1. Breakable Ru complex for bead ECL signal enhancement

The present dataset includes data generated as part of the ECLipse project, focusing on the electrochemiluminescence (ECL) behavior of magnetic microbeads covalently functionalized with a stimuli-responsive derivative of [Ru(bpy)3]2+. The reduction-sensitive disulfide bridge, that links the luminophore to the bead, gets cleaved upon reaction with the neutral TPrA radical, releasing the luminophore in solution and activating the homogeneous mechanism. These microbeads were studied under a microscope on glassy carbon (GC) and Pt substrates at different concentrations of TPrA. Further experiments were conducted on a different kind of beads, that mimick a real bead-based immunoassay. Their ECL response to progressively higher concentration of Ru(II) labels was assessed to simulate calibration curves on commercial immunoassays.

本数据集包含ECLipse项目生成的部分数据，聚焦于经[Ru(bpy)3]²⁺刺激响应性衍生物共价功能化的磁性微珠的电化学发光（ECL）行为。连接发光团与微珠的还原敏感型二硫键在与中性三丙胺（TPrA）自由基反应时发生断裂，使发光团释放至溶液中并激活均相机制。研究人员在显微镜下于玻碳（GC）和铂（Pt）基底上，针对不同TPrA浓度下的这些微珠展开了研究。此外，还对另一种模拟真实基于微珠免疫分析的微珠进行了实验，评估其对逐步升高浓度的钌（II）标记物的ECL响应，以模拟商用免疫分析中的校准曲线。