Data underlying Chapter 2 of PhD thesis: Filament mediated negative feedback regulation of phospholipid synthesis sufficient for coupling growth and membrane synthesis in Escherichia coli

This collection of microscopy images, datasets, and analysis scripts supports an investigation of PlsB regulation and localization in <em>Escherichia coli</em>. The goal of this research is to establish if filamentation of PlsB mediated by membrane abundance is the regulatory mechanism by which the E. coli cell regulates phospholipid synthesis. The growth rate dataset establishes that PlsB-msGFP2 expression does not significantly influence cellular physiology under varied carbon sources. Multiple microscopy datasets capture static and time-lapse fluorescence imaging of <em>E. coli</em> under diverse metabolic, antibiotic, and mutant conditions, revealing how PlsB localization and clustering respond to perturbations. The accompanying MATLAB pipelines enable reproducible quantification of foci number, spatial distribution, and cell morphology from both single timepoint and timelapse experiments. Complementary proteomics measurements quantify PlsB peptide abundance in wild-type and msGFP2-tagged strains. Together, these datasets and analytical tools form an integrated framework for studying the coupling between growth, lipid synthesis, and membrane-associated protein organization in <em>E. coli</em>.

本套显微镜图像、数据集及分析脚本集合，为针对大肠杆菌（Escherichia coli）中PlsB的调控机制与定位特征的研究提供了支撑。本研究的核心目标为验证：由膜丰度介导的PlsB丝状化，是否即为大肠杆菌调控磷脂合成的核心调控机制。生长速率数据集证实，在不同碳源条件下，PlsB-msGFP2的表达不会对大肠杆菌细胞的生理状态产生显著影响。多组显微镜数据集涵盖了大肠杆菌在多种代谢扰动、抗生素处理及突变菌株条件下的静态与延时荧光成像结果，揭示了PlsB的定位模式与聚集特征如何响应外界干扰。配套的MATLAB分析流程，可实现对单时间点与延时实验中荧光焦点数量、空间分布及细胞形态的可重复定量分析。配套的蛋白质组学检测可定量分析野生型与msGFP2标记菌株中PlsB肽段的丰度。综上，本数据集与分析工具共同构建了一套完整的研究框架，用于探究大肠杆菌生长、脂质合成与膜相关蛋白组织之间的耦合关联。