Single-cell RNA-sequencing of Mettl14 conditional knock-out retinas and matched controls

Post-transcriptional m6A methylation on mRNA plays a key role in neural development. Here, we specifically depleted Mettl14 (a key component of m6A complex) in retina progenitor cells by crossing Mettl14 fl/fl mice with the Chx10-Egfp/Cre mouse line and investigated cell type and transcriptome changes in the developing retinas using scRNA-seq. Retinas of Mettl14 fl/fl;Chx10-Egfp/Cre (Mettl14-cKO) mice and age matched controls were collect at P7 and applied to scRNA-seq.信使RNA（messenger RNA, mRNA）的转录后m6A甲基化在神经发育过程中发挥关键调控作用。本研究通过将Mettl14（m6A甲基化复合物的核心组分）纯合条件性敲除小鼠（Mettl14 fl/fl）与Chx10-Egfp/Cre小鼠品系杂交，在视网膜祖细胞中特异性敲除Mettl14；随后借助单细胞RNA测序（scRNA-seq），对发育阶段视网膜的细胞类型及转录组变化展开系统性探究。我们于出生后第7天（P7）采集Mettl14 fl/fl;Chx10-Egfp/Cre（Mettl14条件性敲除，Mettl14-cKO）小鼠及其年龄匹配对照组小鼠的视网膜组织，用于scRNA-seq测序分析。