TBC1D3 Associates with the Histone Lysine N-methyltransferase G9a to Affect the Promotion of Neural Progenitors and Cortical Expansion in an Epigenetic Way [RNA-Seq]

we conducted RNA sequencing using human cerebral organoids treated with TAT peptides Human cerebral organoids were treated with TAT peptides for two groups, that is, TAT-Scrambled (Short for TAT-Scr) with the sequence of YGRKKRRQRRR-FRVRYWFQGCHSEDPWR and TAT-465-481, a special peoptide designed for inhibition of TBC1D3, with the sequence of YGRKKRRQRRR-EGPWFRHYDFRQSCWVR. After 4 days incubation, human cerebral organoid tissues were extracted at cultured day 16 and bulk RNA from TAT-Scr group or TAT-465-481 group was extracted to be sequenced.