Attenuation of progesterone driven mammary stem cells population by telapristone acetate
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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http://data.iscr.ac.cn/Article?id=097ac3e163855d40efe279ff464d199c
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资源简介：
ovariectomized mice treated with E2+Progesterone, E2+Progesterone+TPA, E2+Progesterone+RU486 and sham for 14 days, followed by isolation of luminal mature, luminal progenitor and mammary stem cells through FACS and RNASeq performed for each cell lineage population. TPA and RU486 treated mice show significant decline in mammary stem cell pool pulation comapared to EP treated mice
将去卵巢小鼠分为四组，分别给予雌二醇（E2）+孕酮（Progesterone）、E2+孕酮+十四烷酰佛波醇乙酸酯（TPA）、E2+孕酮+米非司酮（RU486）处理，并设置假手术组，连续处理14天；随后通过荧光激活细胞分选术（FACS）分离腔成熟细胞、腔祖细胞与乳腺干细胞，并对各细胞谱系群体开展RNA测序（RNASeq）。相较于E2+孕酮处理组小鼠，经TPA与RU486处理的小鼠乳腺干细胞池种群数量显著降低。
提供机构：
Northwestern University
创建时间：
2022-02-20
