Data underlying the publication "Equibiaxial and uniaxial cyclic strain similarly affect Notch signaling and vascular smooth muscle cell phenotype in 2D"

This study explored how strain anisotropy affects mechanosensitive Notch signaling and the resulting phenotype of vascular smooth muscle cells (VSMCs). We applied cyclic strains within the physiological range to compare the effects of equibiaxial and uniaxial loading on Notch activity and phenotypic changes in both synthetic and contractile VSMCs. By assessing three deformation parameters, we aimed to identify the main strain feature influencing these responses. Our findings indicate that VSMCs react similarly to both strain types, suggesting that strain anisotropy may not have a significant impact on Notch signaling and phenotype modulation.<br>This dataset contains the videos and data for strain analysis, immunofluorescence staining images, and real-time polymerase chain reaction data.

本研究探讨了应变各向异性（strain anisotropy）对机械敏感性Notch信号通路及血管平滑肌细胞（vascular smooth muscle cells，VSMCs）表型的影响。我们施加生理范围内的周期性应变，对比等双轴加载与单轴加载对合成型及收缩型血管平滑肌细胞的Notch活性与表型改变的作用。通过评估三类形变参数，本研究旨在明确调控上述细胞应答的核心应变特征。研究结果表明，血管平滑肌细胞对两种应变类型的响应无显著差异，提示应变各向异性可能不会对Notch信号通路调控与表型调节产生显著影响。
本数据集包含用于应变分析的视频素材、免疫荧光染色图像，以及实时聚合酶链式反应（real-time polymerase chain reaction）实验数据。