Transcriptome characterization of murine ventricular cardiomyocytes

We collected comprehensive RNAseq data from wildtype murine ventricular cardiomyocytes. Moreover, a cardiomyocyte-specific knockout mouse model for the ion channel regulator CASK was included, since CASK can translocate to the nucleus and regulate gene expression. Knockout models for SAP97 and dystrophin, two ion channels regulators, were also investigated. Sequencing of cardiomycyte RNA from WT mice as well as CASK KO, SAP97 KO and MDX mice. Three replicated experiments were performed for CASK KO and SAP97 KO conditions, and five replicated experiments for the MDX condition.本研究收集了野生型小鼠心室心肌细胞的全套RNA测序（RNAseq）数据。此外，纳入了针对离子通道调控因子CASK的心肌细胞特异性敲除小鼠模型，因CASK可转位至细胞核并调控基因表达。另外还对另外两种离子通道调控因子SAP97与肌营养不良蛋白（dystrophin）的敲除模型开展了研究。对野生型（WT）小鼠、CASK敲除（CASK KO）、SAP97敲除（SAP97 KO）以及MDX小鼠的心肌细胞RNA进行了测序。CASK KO与SAP97 KO组各设置3次生物学重复实验，MDX组则设置5次生物学重复实验。