RNA-seq of primary cardiomyocytes under ischemia-reperfusion injury reveals altered expression in DNA damage repair genes including Xrcc6

To investigate the molecular response of cardiomyocytes to ischemia-reperfusion (I/R) injury, we established an in vitro I/R model using primary neonatal mouse cardiomyocytes. Oxidative stress was simulated by treating the cells with 100 uM hydrogen peroxide for 24 hours, followed by a 24-hour recovery period in complete medium to mimic the ischemia and reperfusion phases, respectively. Bulk RNA sequencing was performed on both control (n = 3) and I/R-treated (n = 4) cardiomyocytes. Transcriptomic profiling identified differentially expressed genes and enriched pathways associated with oxidative stress response, DNA damage repair, and regulation of cell death. This dataset provides valuable insights into the molecular mechanisms underlying myocardial I/R injury and may help identify potential therapeutic targets.