Optimization of Hydrolase Production (Dataset).pdf

The saccharification of biomass with hydrolases from fungi is considered as an efficient and sustainable approach to obtain fermentable sugars. The expression of these hydrolytic enzymes by fungi is mainly by a substrate-induced mechanism, which depends on prevailing environmental conditions. <i>Trametes polyzona, </i>a white rot fungus, is regarded as one of the most efficient producers of hydrolases for the hydrolysis of biomass. This study sought to examine and optimize cultivation conditions for the efficient production of hydrolases by <i>Trametes polyzona </i>(<i>T. polyzona</i>)<i>. </i>Hydrolase production by <i>T. polyzona</i> was examined using parametric optimization. Crude enzyme extracts obtained under varying cultivation conditions were profiled for endoglucanase, betaglucosidase, exoglucanase, xylanase and amylase activities to determine the optimal conditions for hydrolase production. Maximum hydrolase production was achieved by solid-state cultivation of <i>T. polyzona</i> on cassava peels at a moisture content of 60%, pH 6, supplemented with 0.3% urea and 0.03% Mg²⁺, and incubated at 30 ^oC for 14 days. A solid to liquid ratio of 1:2 was found to be ideal for the solid-liquid extraction of enzymes. This study therefore proves that optimization of cultivation conditions is an efficient approach to enhance the production of hydrolases required to breakdown lignocellulosic biomass by <i>T. polyzona.</i>

利用真菌来源水解酶进行生物质糖化被视为获取可发酵糖的高效且可持续的技术路径。真菌所产的这类水解酶的表达主要通过底物诱导机制实现，该机制受当前环境条件的影响与调控。白腐真菌聚芝栓孔菌（Trametes polyzona）被认为是生物质水解用水解酶的最高效生产菌株之一。本研究旨在探究并优化聚芝栓孔菌（T. polyzona）高效产水解酶的培养条件。本研究采用参数优化法对聚芝栓孔菌产水解酶的情况进行考察：对不同培养条件下获得的粗酶提取物进行内切葡聚糖酶、β-葡糖苷酶、外切葡聚糖酶、木聚糖酶与淀粉酶活性分析，以确定产水解酶的最优培养条件。实验结果表明，聚芝栓孔菌以木薯皮为底物进行固态培养时，在含水量60%、初始pH 6、添加0.3%尿素与0.03%镁离子（Mg²+）、30℃下培养14天，可实现水解酶的最大产量。研究发现固液比1:2是酶的固液提取的理想比例。综上，本研究证实，优化培养条件是提升聚芝栓孔菌降解木质纤维素生物质所需水解酶产量的有效手段。