Probiotic Bacillus subtilis Protects against a-Synuclein Aggregation in C. elegans (fluorescence microscopy data)

This project has been submitted by the Maria Doitsidou Lab.

Project contents:
This project contains datasets of z-stack images of C. elegans strains used to study how the gut microbiome affects Parkinson’s disease. Each strain contains a chromosomal insertion containing YFP fused to α-synuclein (pkIs2386[Punc-54::α-synuclein::YFP + unc-119(+)]). The following C. elegans strains were used and/or created for this project:
NL5901 pkIs2386[Punc-54::α-synuclein::YFP + unc-119(+)]
MDH586 daf-2(e1370) III; pkIs2386
MDH585 daf-16(mu86) I; pkIs2386
MDH587 hsf-1(sy441) I; pkIs2386
MDH657 daf-2(e1370) III; daf-16(mu86) I; pkIs2386
MDH614 daf-2(gk390525) III; pkIs2386
MDH611 eat-2(ad465) II; pkIs2386
MDH711 lagr-1(gk331) I, pkIs2386
MDH725 sptl-3(ok1927) II; pkIs2386
MDH724 asm-3(ok1744) IV; pkIs2386.

High magnification (40x objective) z stack images of the head region were obtained by using a Zeiss Axio imager 2 microscope.

Aim:
Study how a probiotic B. subtilis strain affects alpha-synuclein protein aggregation.

Main results:
The authors showed that the probiotic B. subtilis strain PXN21 inhibits and clears a-synuclein aggregation in a C. elegans model. The bacterium acts via metabolites and biofilm formation to activate protective pathways in the host, including DAF-16/FOXO and sphingolipid metabolism.

Contributors:
Maria Eugenia Goya, Feng Xue, Cristina Sampedro-Torres-Quevedo, Sofia Arnaouteli, Lourdes Riquelme-Dominguez, Andres Romanowski, Jack Brydon, Kathryn L. Ball, Nicola R. Stanley-Wall and Maria Doitsidou

These datasets were used in the following publication:

Probiotic Bacillus subtilis Protects against a-Synuclein Aggregation in C. elegans

Maria Eugenia Goya, Feng Xue, Cristina Sampedro-Torres-Quevedo, Sofia Arnaouteli, Lourdes Riquelme-Dominguez, Andres Romanowski, Jack Brydon, Kathryn L. Ball, Nicola R. Stanley-Wall and Maria Doitsidou

Cell Reports January 14, 2020 30 367-380; first published January 14, 2020 https://doi.org/10.1016/j.celrep.2019.12.078