The Evolving Immune Microenvironment of B-cell Acute Lymphoblastic Leukemia [murine dataset]

Single-cell RNA-seq analysis of murine bone marrow and peripheral blood mononuclear cells (PBMCs) immune populations isolated from B-ALL recipient animals and two healthy littermate controls. Overall design: scRNA-Seq: For healthy recipients, processed bone marrow or peripheral blood cells were separated into GFP-CD45+Ly-6G- non-granulocytic cells and GFP-CD45+Ly-6G- non-granulocytic cells, then mixed at a ratio of 8 parts GFP-CD45+Ly-6G- non-granulocytic cells and 1 part GFP-CD45+Ly-6G+ granulocytic cells. For B-ALL recipients, cells were sorted and mixed to give 1 part GFP+CD19+ B-ALL blasts, 8 parts GFP-CD45+Ly-6G- non-granulocytic cells, and 1 part GFP-CD45+Ly-6G+ granulocytic cells.