Effects of pasteurized Akkermansia muciniphila on fecal microbiota composition

The microbial community composition in feces was examined through 16S rRNA sequencing. Supplementation with pasteurized A.muciniphila had no impact on the diversity and richness of commensal bacteria in hyperuricemia mice, as evidenced by the Simpson and Chao1 indices. Phylum-level analysis revealed Bacillota and Bacteroidota as the dominant taxa, with a notable increase in the proportion of Bacteroidota in hyperuricemia mice. Interestingly, Bacteroidota in AKK-treated mice were less abundant compared to the HUA group, approaching the levels observed in the CON group. LEfSe analysis revealed that intervention group receiving pasteurized A.muciniphila displayed a higher abundance of Ileibacterium, Bacteroides, Akkermansia, Verrucomicrobiia, Bifidobacterium, Faecalibacillus. These bacteria have important potential roles in intestinal health, immune system modulation, and metabolic processes.

粪便中的微生物群落组成通过16S rRNA测序（16S rRNA sequencing）进行检测。补充巴氏杀菌的A. muciniphila（Akkermansia muciniphila）对高尿酸血症小鼠共生细菌的多样性和丰富度无显著影响，这一结果由Simpson指数（Simpson index）和Chao1指数（Chao1 index）得以证实。门水平分析显示，厚壁菌门（Bacillota）和拟杆菌门（Bacteroidota）是优势类群，高尿酸血症小鼠中拟杆菌门的比例显著增加。有趣的是，与HUA组相比，AKK处理组小鼠的拟杆菌门丰度更低，接近CON组的水平。LEfSe分析（LEfSe analysis）显示，接受巴氏杀菌A. muciniphila干预的组中，Ileibacterium、Bacteroides、Akkermansia、Verrucomicrobiia、Bifidobacterium和Faecalibacillus的丰度更高。这些细菌在肠道健康、免疫系统调节和代谢过程中具有重要的潜在作用。