Novel Isoforms of the Epigenetic Regulator TET1 Differentially Control CNS Synaptic Function, Plasticity, and Cognition. Novel Isoforms of the Epigenetic Regulator TET1 Differentially Control CNS Synaptic Function, Plasticity, and Cognition

Two distinct Tet1 isoforms are expressed, one encoding full-length TET1 (Tet1L) and the other a truncated transcript (Tet1S), lacking a large portion of the N-terminus. Each isoform displays unique transcriptional regulation, subcellular protein localization and enzymatic activity. In neurons, Tet1L and Tet1S govern the expression and methylation levels of distinct gene ensembles that when disrupted. Overall design: In mouse hippocampal cultured neurons, Tet1L and Tet1S were selectively overexpressed and downregulated to identify loci of differential DNA methylation and gene expression.