Data supplementary to the PhD Dissertation: Driving asymmetric biocatalysis through cofactor regeneration

The enclosed data is complementary to the dissertation “Driving asymmetric biocatalysis through cofactor regeneration”. The aim of the dissertation is to promote biocatalytic asymmetric alkylation and epoxidation by exploring the regeneration of their associated cofactors. To drive asymmetric alkylation, we employed protein engineering to expand the substrate scope of the halide methyltransferase from <em>Arabidopsis thaliana</em> to regenerate the cofactor <em>S</em>-Adenosyl-L-homocysteine with alkyl groups lager than methyl. Asymmetric epoxidation was explored by characterisation of the substrate scope of styrene monooxygenases, and by recycling their cofactor flavin adenine dinucleotide (FAD) in a clean fashion by hydrogenases. The raw data for the figures and tables are sorted per chapter in the accompanying .zip file. For more details on the research objective, type of research and method of data collection, please go to README file provided per chapter.

随附数据是对博士论文《通过辅因子再生驱动不对称生物催化》的补充。本博士论文的研究目标为，通过探究相关辅因子的再生途径，推动生物催化不对称烷基化与环氧化反应的发展。为推进不对称烷基化反应，我们采用蛋白质工程手段，拓展了源自拟南芥（Arabidopsis thaliana）的卤代甲烷甲基转移酶的底物谱，使其可再生带有比甲基更大的烷基链的辅因子S-腺苷-L-高半胱氨酸（S-Adenosyl-L-homocysteine）。针对不对称环氧化反应，我们通过表征苯乙烯单加氧酶的底物谱，并借助氢化酶以洁净环保的方式循环再生其辅因子黄素腺嘌呤二核苷酸（FAD），开展了相关研究。本随附压缩包内，所有图表对应的原始数据均按章节进行分类整理。若需了解本研究的研究目标、研究类型以及数据采集方法的更多细节，请查阅各章节附带的README文件。