Farrell AJP_cell Supplemental figure 1.pdf

Expression of the microRNA-17~92 cluster is up-regulated by aldosterone in mouse cortical collecting duct principal cells, exclusively in female mice. MiR-19 in this cluster targets the serum and glucocorticoid kinase (SGK1) to downregulate both mRNA and protein expression, resulting in a decrease in sodium transport across epithelial cells of the collecting duct. The miR-17~92 cluster is evolutionarily conserved and may act as a novel feedback regulator for aldosterone signaling in females. This supplement contains the species sequences used for phylogenetic analysis.

小鼠皮质集合管主细胞中，醛固酮可上调miR-17~92基因簇（microRNA-17~92 cluster）的表达，且该调控仅特异性地存在于雌性小鼠体内。该基因簇中的miR-19可靶向血清和糖皮质激素激酶（serum and glucocorticoid kinase, SGK1），下调其mRNA与蛋白的表达水平，进而降低集合管上皮细胞的跨上皮钠转运能力。miR-17~92基因簇具有进化保守性，可作为雌性小鼠体内醛固酮信号通路的新型反馈调节因子。本补充数据集包含系统发育分析所用的物种序列。