Trametes lactinea and T. villosa collected in Brazil are able to discolor indigo carmine

ABSTRACT Dyes used in the textile industry contribute significantly to the increase of water pollution as they are disposed of, most of the time, without proper treatment. Indigo carmine is a synthetic dye widely used in the coloring of jeans and is considered difficult to remove, causing irreversible damage to the food chain in ecosystems. Mycomediation appears as an economical and sustainable way to treat textile effluents, and this work tested three strains of Trametes collected in Brazil against the ability to discolor the indigo carmine and also the activity of laccase, lignin and manganese peroxidases. The experiment was carried out in Kirk medium under static, non-sterile condition, at ± 28 °C for 120 h. Trametes lactinea (URM8350) discolored 81.40 % of the indigo carmine, T. lactinea (URM8350) 85.09 % and T. villosa (URM8022) 96.11 %. Laccase was detected in all specimens. Manganese peroxidase was detected in T. villosa and T. lactinea (URM8354), while lignin peroxidase was not detected in any of the isolates. The ability of T. lactinea to discolor dyes is reported for the first time. The discoloration rates demonstrate the ability of the strains to discolor carmine indigo and their promising use in the discoloration processes in wastewater from the textile segment.

摘要：纺织工业用染料在排放时大多未经妥善处理，是造成水体污染加剧的重要诱因。靛蓝胭脂红（indigo carmine）是一种广泛用于牛仔布染色的合成染料，因其难以被去除，会对生态系统的食物链造成不可逆损害。真菌修复技术（mycomediation）作为一种经济且可持续的纺织废水处理方案，本研究选取了在巴西采集的3株栓菌属（Trametes）菌株，测试其对靛蓝胭脂红的脱色能力以及漆酶（laccase）、木质素过氧化物酶（lignin peroxidase）和锰过氧化物酶（manganese peroxidase）的活性。实验在柯克培养基（Kirk medium）中开展，采用静态非无菌条件，培养温度控制在±28℃，培养时长120小时。结果显示，乳栓菌（Trametes lactinea，URM8350）的靛蓝胭脂红脱色率为81.40%，乳栓菌（Trametes lactinea，URM8354）为85.09%，毛栓菌（Trametes villosa，URM8022）可达96.11%。所有供试菌株均检测到漆酶活性；毛栓菌与乳栓菌（URM8354）中可检测到锰过氧化物酶活性，但所有菌株均未检测到木质素过氧化物酶活性。本研究首次报道了乳栓菌具备染料脱色能力。上述脱色率证实了这些菌株对靛蓝胭脂红的脱色潜能，表明其在纺织行业废水脱色工艺中具有良好的应用前景。