Arabidopsis PSKR1 mutants rhizosphere microbiome sequencing

This is the raw 16S rRNA sequencing dataset for the rhizosphere microbiome community of Arabidopsis genotype Col-0, pskr1-3, hsm7, 35S:PSKR1-GFP as well as bulk soil samples. We have sequenced 10 biological replicates for each genotype, with rhizosphere soil samples harvested from 4 plants as one replicate.Microbiome members provide various benefits to their plant hosts, such as growth promotion and induced defense responses; however, how plants maintain an appropriate defense level towards the rhizosphere microbial community while coordinating the promoted growth is not well understood. Here we show that the Arabidopsis PSKR1(PHYTOSULFOKINE RECEPTOR 1) loss-of-function mutants display an autoimmune phenotype with growth-promoting bacteria P. fluorescens inoculation and harbour a markedly reduced Pseudomonas relative abundance in the rhizosphere microbiome. RNAseq transcriptional profiling and epistasis analysis demonstrated a crucial role of PSKR1 in regulating plant growth-defense trade-off during plant-microbiome interaction; PSKR1 upregulates plant photosynthesis and root growth but suppresses salicylic acid-mediated defense responses. Finally, GUS staining experiments showed that P. fluorescens induces PSKR1 expression in roots, suggesting P. fluorescens might manipulate plant signaling to promote its colonization. Here we highlight the key role of PSKR1 in enriching beneficial bacteria P. fluorescens in the rhizosphere and helping plants control growth-defense balance for maintaining plant fitness in a microbe-enriched environment.