Amplicon sequencing of Cyp51 alleles from Pyrenophora teres using the Oxford Nanopore Technologies MinION (version 2)

Data description: Samples are barley (Hordeum vulgare) leaves infected with Pyrenophora teres collected from paddocks in Western Australia. The azole fungicide target gene Cyp51 was amplified from the samples using unique molecular identifiers according to Zulak et al., (2024) Scientific Reports 14:1 6285. Amplicons were subsequently sequenced with the Oxford Nanopore Technologies MinION sequencer using the Native Barcoding Kit V14 and the MIN114 flow cell. The resulting data file is a .fastq file containing sequence information.Collected under CCDM Project A3.1
Number of files/types: 1 .zip file containing 286 .fastq.gz filesGeographic information (if relevant): Western AustraliaKeywords: sequences, genes, fungal

This is the updated October 2025 dataset version.

数据说明：本数据集样本采自澳大利亚西部农田，为感染网斑核腔菌（Pyrenophora teres）的栽培大麦（Hordeum vulgare）叶片。参照Zulak等人2024年发表于《Scientific Reports》14卷1期第6285页的研究方案，研究人员通过唯一分子标识符（unique molecular identifiers）从样本中扩增得到唑类杀菌剂靶标基因Cyp51。随后使用Oxford Nanopore Technologies MinION测序仪，搭配Native Barcoding Kit V14建库试剂盒与MIN114测序流槽，对扩增子进行测序。最终产出的数据文件为包含序列信息的.fastq格式文件，本次样本采集工作依托CCDM项目A3.1开展。
文件数量与类型：1个.zip格式压缩包，内含286个.fastq.gz格式文件。
地理信息（如适用）：澳大利亚西部
关键词：序列、基因、真菌

本数据集为2025年10月更新版本。