Supplementary Material for: Automated Spectral EEG Analyses of Premature Infants during the First Three Days of Life Correlated with Developmental Outcomes at 24 Months

<b><i>Background:</i></b> Spectral EEG analysis using automated quantification of total absolute band power (tABP) for long-term brain monitoring is reliable. We hypothesised that tABP during the first critical days of life could be a useful tool for predicting later developmental outcomes. <b><i>Objective:</i></b> To determine whether measuring EEG background activity in premature infants with automated tABP quantification during the first 3 days of life correlated with their developmental outcomes at 24 months. <b><i>Methods:</i></b> Preterm infants (group 1, gestational age, GA 24–28 weeks and group 2, GA 28–31 weeks) were continuously monitored by EEG for 3 days after birth. Their developmental outcomes were assessed using the Bayley-II and Peabody-2 developmental tests at 24 months. Their respective indices were calculated. Normal (index ≥85) and abnormal (index &lt;85) outcomes were correlated with the tABP. <b><i>Results:</i></b> In group 1, the tABP was significantly lower in the abnormal infants than in the normal infants. The specificity and negative predictive value were also high for all of the tests that were applied in this group. In group 2, there was no correlation between the tABP and developmental outcome. <b><i>Conclusion:</i></b> This study found that extremely premature infants with poor developmental outcomes had significantly lower tABP values in their first days of life compared to infants from the same group with normal outcomes. This method may be useful in predicting later outcomes in extremely premature infants and has the advantage of being automated.

**背景**：采用总绝对频带功率（total absolute band power, tABP）自动量化的脑电频谱分析应用于长期脑监测具有良好可靠性。本研究假设，新生儿出生早期关键几日的tABP可作为预测远期发育结局的有效工具。
**目的**：明确出生前3日内采用自动tABP量化方法对早产儿脑电背景活动进行检测的结果，是否与其24月龄时的发育结局存在相关性。
**方法**：本研究纳入两类早产儿：第1组胎龄（gestational age, GA）24~28周，第2组GA 28~31周，所有受试者于出生后连续接受3日脑电监测。于受试者24月龄时，采用贝利婴儿发育量表第二版（Bayley-II）与皮博迪发育量表第二版（Peabody-2）评估其发育结局，并计算对应发育指数。将发育指数≥85归为正常结局，指数<85归为异常结局，分析两类结局与tABP的相关性。
**结果**：第1组中，发育结局异常的早产儿其出生早期tABP水平显著低于结局正常的受试者；本研究针对该组开展的所有检测指标，其特异性与阴性预测值均较高。第2组中，tABP水平与发育结局未表现出相关性。
**结论**：本研究发现，与同组内发育结局正常的早产儿相比，远期发育结局不佳的极早早产儿，其出生早期的tABP水平显著更低。该自动量化方法可用于预测极早早产儿的远期发育结局，具备自动化操作的显著优势。