SH1038748.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE 提供了一套统一的方法，用于界定、识别、传播以及处理基于DNA的物种假说（Species Hypotheses，以下简称SH）。国际核酸序列数据库中的所有真菌内转录间隔区（Internal Transcribed Spacer，简称ITS）序列，均通过应用一系列动态距离阈值（区间为0.5%以下至3.0%）聚类至近似物种水平。所有SH均会被赋予以数字对象标识符（Digital Object Identifier，简称DOI）形式存在的唯一稳定名称，其分类学与生态学注释均通过分布式的网页端第三方注释工作完成验证。系统会结合每个SH内所有序列的鉴定结果，尽可能为其关联对应的分类单元名称及分类层级（如门、纲、目等）。系统会为每个SH选取一条自动或人工指定的序列作为代表序列。此类代表序列已发布于 https://unite.ut.ee/repository.php，可供科研社区用于本地序列相似性搜索、新一代测序分析流程等场景。随着公共真菌ITS序列数量的增长，该系统与配套数据将自动完成更新。