PMACS-11.

<b>Project title</b>: PMACS-11<b>Grant Information:</b> AHA Grant 9TPA34910007, Regulatory role of pericardial macrophages in myocardial infarction<b>Project Description:</b>· Animal Model: Myocardial Infarction· Animal Age: 8-12 Weeks· Animal Sex: Male· Animal Strain: C57B6/J· Number: 9 mice· Groups: 5 chlodronate liposome injection, 4 empty liposome injections, all infarctions· Experimental Conditions: Mice were given MI via LAD ligation alongside intrapericardial injection of chlodronate loaded liposomes. Animals were sacrificed at day 21 post-infarction or sham surgery. Animals were analyzed via EKG and then hearts were harvested for flow cytometry and Mason’s Trichrome histology<b>Code/Scripts: </b>N/A<b>Licenses: </b>N/A<br><br><br><br><br><br><br>

<b>项目名称</b>：PMACS-11
<b>资助信息</b>：美国心脏协会（American Heart Association, AHA）资助项目编号9TPA34910007，研究主题为「心包巨噬细胞在心肌梗死中的调控作用」
<b>项目描述</b>：
· 动物模型：心肌梗死模型
· 动物周龄：8~12周
· 动物性别：雄性
· 动物品系：C57B6/J
· 小鼠数量：9只
· 分组：5只接受氯膦酸盐脂质体注射，4只接受空白脂质体注射，所有小鼠均构建心肌梗死模型
· 实验方案：小鼠通过左前降支（Left Anterior Descending artery, LAD）结扎诱导心肌梗死，同时于心包腔内注射负载氯膦酸盐的脂质体；于造模后21天或假手术后处死动物。先通过心电图（electrocardiogram, EKG）完成检测分析，随后摘取心脏，采用流式细胞术（flow cytometry）及马松三色（Mason’s Trichrome）组织病理学方法进行检测分析
<b>代码/脚本</b>：无（N/A）
<b>许可协议</b>：无（N/A）