Maize cytoplasmic male sterility (CMS) S-type restorer gene Rf3

A newly identified protein that is encoded by a polynucleotide sequence associated with cytoplasmic male sterility restorer activity (Rf3) is described. The cytoplasmic male sterility restorer gene can be inserted through breeding introgression into plant genomes to restore cytoplasmic male sterility in plants. Further applications of the newly identified polynucleotide sequence associated with cytoplasmic male sterility restorer activity include a mutation (rf3) which results in cytoplasmic male sterility. The cytoplasmic male sterility restorer gene can be inserted through breeding introgression into plant genomes to result in cytoplasmic male sterility in plants. Methods for detecting the cytoplasmic male sterility restorer (Rf3) and the cytoplasmic male sterility (rf3) gene sequences are further described.

本研究报道了一种新鉴定得到的蛋白质，该蛋白质由与细胞质雄性不育（cytoplasmic male sterility）恢复活性相关的多核苷酸序列编码，该序列对应的恢复基因为Rf3。该细胞质雄性不育恢复基因可通过育种渐渗的方式插入植物基因组，以恢复植物的细胞质雄性不育性状。上述与细胞质雄性不育恢复活性相关的新鉴定多核苷酸序列，其进一步应用包含可诱导细胞质雄性不育的突变体rf3。该细胞质雄性不育恢复基因还可通过育种渐渗的方式插入植物基因组，从而使植物产生细胞质雄性不育性状。本研究还描述了用于检测细胞质雄性不育恢复基因Rf3以及细胞质雄性不育基因rf3序列的方法。