TLR9 re-expression in cancer cells extends the S-phase and stabilizes p16INK4a protein expression.. Homo sapiens

Using Illumina HumanHT-12 v4 Expression BeadChips, providing a genome-wide coverage of over 24,000 genes, we have analysed gene expression variation in HNSCC 136 cells transduced with pLVUT’ TLR9 or GFP where the same number of cells were harvested and RNA extracted after 36 h treatment with doxycyline. Overall design: Total RNA obtained HNSCC 136 cells transduced with pLVUT’ TLR9 or GFP as controls where the same number of cells were harvested and RNA extracted after 36 h treatment with doxycyline.Diffrential expression analysis was conducted with GFP replicates as reference group.