Gene expression changes in galls at 21 dai and corresponding uninfected roots from Poplar CAD and WT lines

Illumina technology was used to generate mRNA profiles of galls from root-knot nematodes infected and corresponding uninfected roots from Poplar CAD and WT lines. RNA was extracted from 3 replicates.TruSeq mRNA Stranded libraries were constructed and after pooling and normalization of libraries, sequencing was done on a NextSeq500 Sequencing System. Raw reads were trimmed for quality and mapped to the substituted genome sequence of P. tremula x P. alba 717-1B4 using CLC Genomics Workbench v9.5.2 and the primary transcripts only. mRNA profiles of galls and uninfected roots from Poplar CAD and WT lines were generated by NextSeq500 sequencing (2x75bp). Three biological replicates were sequenced.