Evaluation of equine aqueous humor protein

ABSTRACT Evaluation of equine aqueous humor (AH) proteins can help the diagnosis of uveitis. However, studies on proteins in this fluid in healthy horses are scarce and present variations according to the methodology employed. This study aimed to perform protein analysis and cytology of equine aqueous humor of healthy horses and verify its correlation with plasmatic proteins. Thirteen adult horses (26 eyes), mixed breed, male or female were evaluated. A volume of 0.5 mL of aqueous humor was collected through aqueocentesis from both eyes. The samples were submitted to protein quantification by modified Bradford method and to sodium dodecyl sulphate - polyacrylamide gel electrophoresis (SDS-PAGE), and to cytological evaluation. Blood was collected for determination of plasmatic protein concentration. Thirteen eyes (50% of the samples) had values larger than zero by the Bradford method, with an average of 40.3 mg/dl±6.45. Electrophoresis showed presence of higher masses of proteins (43 KDa). There were no cells in 96.15% of the samples (25 eyes). Healthy equines presented low protein concentration in the HA. The ratio between protein concentration in the aqueous humor / total plasma protein of 0.56%.

摘要 马房水（aqueous humor, AH）蛋白质检测有助于葡萄膜炎（uveitis）的诊断。然而，针对健康马房水内蛋白质的相关研究较为匮乏，且不同实验方法会导致研究结果存在差异。本研究旨在对健康马的房水开展蛋白质分析与细胞学检测，并验证其与血浆蛋白的相关性。
本研究共纳入13匹成年混血马（共26只眼），雌雄不限。通过房水穿刺术（aqueocentesis）采集双眼各0.5 mL房水样本。对样本采用改良布拉德福德法（Bradford method）进行蛋白质定量，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（sodium dodecyl sulphate - polyacrylamide gel electrophoresis, SDS-PAGE）分析蛋白质组分，并开展细胞学检测。同时采集血液样本以测定血浆蛋白浓度。
经布拉德福德法检测，13只眼（占总样本量的50%）的蛋白质浓度大于0，平均浓度为40.3 mg/dL±6.45。电泳结果显示存在分子量为43 kDa的蛋白质。96.15%的样本（25只眼）未检测到细胞。健康马房水内蛋白质浓度较低，房水与血浆总蛋白的浓度比值为0.56%。