Palaemon serratus metabolome

The prawn Palaemon serratus exhibits a large distribution (occurring along the Northeastern Atlantic coast to the Mediterranean), but presents a small-scale commercial importance, and has thus been found suitable as model organism valuable for various ecotoxicological studies. However, little is still known about the potential input of its metabolome and particularly concerning a potential molecular sexual dimorphism observable in the different tissues of this organism. In an ecotoxicological point of view, inter-sex and inter-organ differences in the metabolome composition might induce bias and influence the robustness of data analysis or interpretation. To explore such possibilities, we obtained qualitative metabolomic data from the analysis of different organs of mature male and female Palaemon serratus. We used ultra-high-performance liquid chromatography-electrospray ionization-high resolution tandem mass spectrometry (UHPLC-ESI-HRMS on positive mode) to characterize the 75%-extracted metabolome of both gills, hepatopancreas, nervous gland, muscle and gonads. The data were dereplicated using specific metabolomic software (MetaboScape 4) and 2,782 features were extracted, 1,720 of them being also analyzed on MS/MS mode, supporting molecular networking investigations with Metgem 1.3.6. These metabolites were thus attemptedly identified using GNPS databases for de-novo annotation followed by manual curation (n=84). This data provides essential information on the important sexual dimorphism occurring at the molecular level in the different organ and supports further research on physiology and ecotoxicology in common European prawn.

斑马虾Palaemon serratus广泛分布于东北大西洋沿岸至地中海地区，但其商业价值相对有限，因而被选作生态毒理学研究中的模式生物。尽管如此，关于其代谢组学的潜在输入以及该生物不同组织中潜在的分子性别二型性了解甚少。从生态毒理学的视角来看，代谢组成分在性别间和器官间的差异可能导致数据分析和解读的偏差，进而影响分析结果的稳健性。为探究此类可能性，我们收集了成熟雄性和雌性斑马虾不同器官的定性代谢组数据。通过超高效液相色谱-电喷雾电离-高分辨率串联质谱(UHPLC-ESI-HRMS，正离子模式)对鳃、肝胰腺、神经腺、肌肉和生殖腺的75%提取代谢组进行表征。利用特定的代谢组学软件(MetaboScape 4)进行去重，共提取2,782个特征，其中1,720个特征在MS/MS模式下进行分析，支持使用Metgem 1.3.6进行分子网络研究。这些代谢物随后尝试使用GNPS数据库进行从头注释，并辅以人工校正（n=84）。这些数据提供了关于不同器官在分子水平上发生的重要性别二型性的关键信息，并支持对欧洲斑马虾生理学和生态毒理学的进一步研究。