miR-1205 promotes apoptosis in osteosarcoma cells by downregulating CYP2C19
收藏资源简介:
This dataset contains Sanger sequencing data used to verify plasmid constructs in the associated manuscript. The deposited files include sequencing trace records for the CYP2C19 pcDNA3.1-3×Flag expression plasmid and the CYP2C19 3′-UTR luciferase reporter vector. The Sanger sequencing results were used to confirm the correct insertion and sequence integrity of the CYP2C19 coding sequence and luciferase reporter constructs before subsequent cell transfection and functional assays. The dataset includes the following files/folders: CYP2C19 pcDNA3.1-3×Flag: Sanger sequencing files for the CYP2C19 overexpression plasmid. Luciferase reporter vector: Sanger sequencing files for the CYP2C19 3′-UTR luciferase reporter constructs. Sanger Sequence trace.docx: Summary of sequencing trace and verification results. These data support the plasmid construction and sequence validation described in the Materials and Methods section of the manuscript.



