Quantitative phytoplankton profiles data for Pensacola Transect Station P7 collected during R/V Bellows cruise BE-1212 in the Gulf of Mexico on 2012-05-05

This dataset reports phytoplankton species abundance data for samples collected on 2012-05-05 onboard the R/V Bellows cruise BE-1212 in the Gulf of Mexico. Samples were taken at Pensacola Transect Station P7 (29.75 degrees N, 87.25 degrees W) in the Florida Panhandle Bight at the head of DeSoto Canyon. One-liter samples were collected from the surface and at depths of 25m, 50m, 75m, 100m, 125m and 150m using Niskin bottles. Samples were filtered onto a 0.45 micrometer nitrocellulose filter and air-dried. A portion of each filter was mounted on an aluminum stub, sputter-coated, and examined with a JEOL JSM-6480 LV scanning electron microscope. Diatoms, coccolithophores, and armored dinoflagellates were identified to the lowest possible taxonomic category. The number of cells of each taxon in a field of view at a magnification of 1000x was recorded, with up to 700 fields-of-view examined, depending on the density of phytoplankton in the sample. The dataset includes the depth, the volume filtered, the number of cells of each taxon counted, and the calculated abundance of each taxon in cells per liter. Micrographs taken during the analysis are included.

本数据集记录了2012年5月5日在墨西哥湾R/V Bellows号科考船航次BE-1212中采集的浮游植物（phytoplankton）物种丰度数据。样品采集于德索托峡谷头部佛罗里达潘汉德尔湾的彭萨科拉断面站P7（北纬29.75°，西经87.25°）。采用尼斯金采水器（Niskin bottles）分别采集表层及25m、50m、75m、100m、125m、150m水深的1升水样。将样品通过0.45微米硝酸纤维素滤膜（nitrocellulose filter）过滤后进行风干处理。取每张滤膜的一部分置于铝样品台上，经喷金镀膜后，使用JEOL JSM-6480 LV型扫描电子显微镜（scanning electron microscope）开展观测。对硅藻（diatoms）、颗石藻（coccolithophores）及被甲甲藻（armored dinoflagellates）进行了尽可能精细的分类阶元鉴定。在1000倍放大倍率下，记录每个分类群在单个视野内的细胞数量；依据样品中浮游植物的密度，最多可观测多达700个视野。本数据集包含采样水深、过滤水样体积、各分类群计数的细胞数，以及以每升细胞数为单位计算得到的各分类群丰度值。数据集同时收录了分析过程中拍摄的显微照片。