Data of the publication "Rapid and Differential Evolution of the Venom Composition of a Parasitoid Wasp Depending on the Host Strain"

Data files for the publication "Rapid and Differential Evolution of the Venom Composition of a Parasitoid Wasp Depending on the Host Strain." ******************************* ****** GLOBAL ANALYSIS ******************************* The compressed folder "./exemple_gel_1.zip" is an example of analysis of a gel done with CLIQS (http://totallab.com/home/cliqs/). "img_7061_converted.tif" is the analysed picture. "IMG_7061.1dxml" is the CLIQS database containing the position of the lanes, the Rf lines and the "grimaces". For details see (1). "export_without_bg" and "export_with_bg" are containing the intensity profiles exported by CLIQS and used as input for the R-functions published in (1). The file "./global_analysis_nobg_H_Q.txt" is the output of the the R-functions published in (1). This is the dataset corresponding to the global analysis of this paper. Column "nom":X[gel number]_[number of the lane in the gel, excluding the number of the lane] Columns 1 to 34: intensity of the reference bands in the corresponding lane (row in the table). Column "replicate": ID of the biological replicate of the experimental evolution. Column "generation": Generation of the experimental evolution, F1 being the hybrids of ISm and ISy. Column "hote": Host from in which the parasitoid developed (YR = resistant; SY = sensible; sen = host on which ISm and ISy are maintained: Nasr) ********************************* ****** SPECIFIC ANALYSIS ********************************* ./specific_analysis_LbGAP2.txt Column "nom": X[gel number]_[number of the lane in the gel, excluding the number of the lane] Column "replicate": ID of the biological replicate of the experimental evolution. Column "generation": Generation of the experimental evolution, F1 being the hybrids of ISm and ISy. Column "hote": Host from in which the parasitoid developed (YR = resistant; SY = sensible; sen = host on which ISm and ISy are maintained: Nasr) Column "LbGAP2_intens_western": Raw intensity of the western-blot against LbGAP2 Column "intens_mediane_global_analysis": Median intensity of the reference bands of the globale analysis used to correct the raw intensity of the western-blot against LbGAP2 (see the material and method). ./specific_analysis_LbGAP_LbSPN.txt Column "nom": X[gel number]_[number of the lane in the gel, excluding the number of the lane] Column "replicate": ID of the biological replicate of the experimental evolution. Column "generation": Generation of the experimental evolution, F1 being the hybrids of ISm and ISy. Column "hote": Host from in which the parasitoid developed (YR = resistant; SY = sensible; sen = host on which ISm and ISy are maintained: Nasr) Column "LbGAP": Presence or absence of the protein LbGAP (LbGAP is a dominant marker) Column "LbSPNm": Presence or absence of the protein LbSPNm (LbSPN is a co-dominant marker) Column "LbSPNy": Presence or absence of the protein LbSPNy (LbSPN is a co-dominant marker) (1) : Mathé Hubert, H., Gatti, J.-L., Colinet, D., Poirié, M., & Malausa, T. (2015). Statistical analysis of the individual variability of 1D protein profiles as a tool in ecology: an application to parasitoid venom. Molecular Ecology Resources, 15, 1120–1132. http://doi.org/10.1111/1755-0998.12389

本数据集对应发表论文"Rapid and Differential Evolution of the Venom Composition of a Parasitoid Wasp Depending on the Host Strain."的配套数据文件。
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****** 全局分析
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压缩文件夹`./exemple_gel_1.zip`为使用CLIQS（http://totallab.com/home/cliqs/）完成的一维凝胶电泳分析示例文件。其中`img_7061_converted.tif`为完成分析的目标图像；`IMG_7061.1dxml`为CLIQS数据库文件，包含泳道位置、Rf值线及条带轮廓（grimaces）。详细说明参见文献(1)。
`export_without_bg`与`export_with_bg`文件夹内含CLIQS导出的强度分布文件，可作为文献(1)中发表的R函数的输入数据。
文件`./global_analysis_nobg_H_Q.txt`为文献(1)中发表的R函数的输出结果，对应本文的全局分析数据集。各字段说明如下：
- 字段"nom"：格式为`X[凝胶编号]_[凝胶内泳道编号（不含泳道序号前缀）]`
- 第1至34列：对应泳道内参考条带的强度值（表格行与泳道一一对应）
- 字段"replicate"：实验演化的生物学重复ID
- 字段"generation"：实验演化的世代数，其中F1为ISm与ISy的杂交后代
- 字段"hote"：寄生蜂发育的宿主类型（YR为抗性宿主；SY为敏感宿主；sen为ISm与ISy的维持宿主：Nasr品系）
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****** 特定分析
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1. `./specific_analysis_LbGAP2.txt`
各字段说明如下：
- 字段"nom"：格式为`X[凝胶编号]_[凝胶内泳道编号（不含泳道序号前缀）]`
- 字段"replicate"：实验演化的生物学重复ID
- 字段"generation"：实验演化的世代数，其中F1为ISm与ISy的杂交后代
- 字段"hote"：寄生蜂发育的宿主类型（YR为抗性宿主；SY为敏感宿主；sen为ISm与ISy的维持宿主：Nasr品系）
- 字段"LbGAP2_intens_western"：针对LbGAP2的蛋白质免疫印迹（Western blot）原始强度值
- 字段"intens_mediane_global_analysis"：全局分析中参考条带的中位强度值，用于校正针对LbGAP2的蛋白质免疫印迹原始强度值（详见材料与方法部分）

2. `./specific_analysis_LbGAP_LbSPN.txt`
各字段说明如下：
- 字段"nom"：格式为`X[凝胶编号]_[凝胶内泳道编号（不含泳道序号前缀）]`
- 字段"replicate"：实验演化的生物学重复ID
- 字段"generation"：实验演化的世代数，其中F1为ISm与ISy的杂交后代
- 字段"hote"：寄生蜂发育的宿主类型（YR为抗性宿主；SY为敏感宿主；sen为ISm与ISy的维持宿主：Nasr品系）
- 字段"LbGAP"：蛋白LbGAP的存在与否（LbGAP为显性标记）
- 字段"LbSPNm"：蛋白LbSPNm的存在与否（LbSPN为共显性标记）
- 字段"LbSPNy"：蛋白LbSPNy的存在与否（LbSPN为共显性标记）

(1) Mathé Hubert, H., Gatti, J.-L., Colinet, D., Poirié, M., & Malausa, T. (2015). 一维蛋白质谱个体变异性的统计分析及其生态学应用：以寄生蜂毒液为例. 分子生态学资源, 15, 1120–1132. https://doi.org/10.1111/1755-0998.12389