Two new species of Parobisium (Pseudoscorpiones: Neobisiidae) Chamberlin, 1930 from South Korea, with a preliminary phylogeny of the genus.

Poorly dispersing pseudoscorpions (Arachnida: Pseudoscorpiones) are often considered suitable models for biogeography studies. Several lineages show interesting distribution patterns, such as the genus Parobisium Chamberlin, 1930 that occurs in the western U.S.A. and eastern Asia but is absent in Europe and central Asia. Here we review the pseudoscorpion genus Parobisium using an integrative taxonomic approach and describe two new species from South Korea based on morphological and molecular data: Parobisium namkungi sp. nov. and P. troglophilum sp. nov. Phylogenetic analyses using three genetic markers, cytochrome c oxidase subunit I (COI), histone H3A, and 28S ribosomal DNA also reveal that two former subspecies, P. magnum ohuyeanum (Morikawa, 1952) and P. magnum chejuense (Morikawa, 1970), are genetically distinct enough to recognize them as separate species, P. ohuyeanum stat nov. and. P. chejuense stat. nov.. Table S1. PCR conditions to generate DNA sequences for “Two new species of Parobisium (Pseudoscorpiones: Neobisiidae) Chamberlin, 1930 from South Korea, with a preliminary phylogeny of the genus”. Table S2. Primers used to generate DNA sequences used for “Two new species of Parobisium (Pseudoscorpiones: Neobisiidae) Chamberlin, 1930 from South Korea, with a preliminary phylogeny of the genus”. Table S3. GenBank accession numbers for DNA sequences of the 28S rDNA (28S), histone H3A (H3), and Cytochrome c Oxidase subunit I (COI) used for “Two new species of Parobisium (Pseudoscorpiones: Neobisiidae) Chamberlin, 1930 from South Korea, with a preliminary phylogeny of the genus”.