Profiling of Granzyme B Secreting B cells

B cells with regulatory functions have been described both in immune homeostasis in healthy volunteers and in different pathological conditions. Whereas Il-10 secreting B cells represent the main subset described, recently, a subset of B cells secreting granzyme B (GZMB+) was evidenced in a growing numbers of immunological contexts, autoimmunity, chronic infections and neoplasias. Until now, no phenotype has been evidenced for these GZMB+ B cells and their regulatory functions as well are still to clarify. Overall design: Purified B cells from human PBMC were differentiate into GZMB secreting cells in vitro. After 3 days of enrichment, induced GZMB+ B cells and GzmB- B cells were sorted.