RNA-seq profiling of foetal-derived Langerhans cells from wildtype and Suz12 knockout mice

Macrophages are at the forefront of immune responses and their transcriptional programs are modified by their tissue environment and in response to immunological challenge. Post-translational modifications of histones, such as histone H3 lysine 27 tri-methylation (H3K27me3) by the polycomb repressive complex 2 (PRC2), are tightly associated with epigenetic regulation of gene expression. To explore whether H3K27me3 is involved in either the establishment or function of the mononuclear phagocyte system, we selectively deleted the SUZ12 gene in one-week old mice, a core component of PRC2. SMART-Seq RNA libraries were generated from 5000 purified Langerhans cells from the skin of one week old wild mice (2 biological replicates) or Suz12cKO (3 biological replicates). Samples were sequenced with an Illumina NextSeq 500 sequencing system, producing between 22-34 million paired-end 75 bp reads per sample.