Experimental horizontal transfer of phage-derived genes to Drosophila confers innate immunity to parasitoids

Metazoan parasites have played an outsized role in shaping innate immunity in animals. Insects are excellent models for illuminating the strategies that animals evolved to neutralize such enemies, including nematodes and parasitoid wasps. One such strategy relies on endosymbioses between insects and bacteria that express phage-encoded toxins as well as horizontal transfer of the genes that encode the toxins to insects. Here, we used genome editing in Drosophila melanogaster to recapitulate the evolution of two of these toxin genes — cytolethal distending toxin B (cdtB) and apoptosis inducing protein of 56kDa (aip56) — that were horizontally transferred likely from phages of endosymbiotic bacteria to insects millions of years ago. We found that a cdtB::aip56 fusion gene (fusionB), which is conserved in D. ananassae subgroup species, dramatically promoted fly survival and suppressed parasitoid wasp development when heterologously expressed in D. melanogaster immune tissues. We found that ..., , , # **Experimental horizontal transfer of phage-derived genes to *Drosophila* confers innate immunity to parasitoids**

[https://doi.org/10.5061/dryad.q573n5tsh](https://doi.org/10.5061/dryad.q573n5tsh)

## Description of the data and file structure

The following repository contains raw data for genomic analyses and count-based experiments described in the manuscript \"Experimental horizontal transfer of phage-derived genes to *Drosophila* confers innate immunity to parasitoids\"

Authors:  Rebecca L. Tarnopol*, Josephine Tamsil, Gyöngyi Cinege, Ji Heon Ha, Kirsten I. Verster, Edit Ábrahám, Lilla B. Magyar, Bernard Y. Kim, Susan L. Bernstein, Zoltán Lipinszki, István Andó, Noah K. Whiteman 

Please cite the associated manuscript: [https://doi.org/10.1016/j.cub.2024.11.071](https://doi.org/10.1101/2024.05.29.596511)

### Files and variables

### File: Fig1\_rawdata.zip

**Description:** Contains raw data for generation of Figure 1 and related supplementary figures 

\> ananassae_final_wh...

后生动物寄生虫（Metazoan parasites）在塑造动物先天免疫（innate immunity）方面发挥了远超预期的关键作用。昆虫是阐明动物演化出的对抗这类天敌（包括线虫（nematodes）与寄生蜂（parasitoid wasps））策略的绝佳模型。其中一类策略依赖于昆虫与细菌的内共生（endosymbioses）关系：这类细菌可表达噬菌体（phage）编码的毒素，并将编码毒素的基因水平转移至昆虫体内。本研究通过对黑腹果蝇（Drosophila melanogaster）进行基因组编辑（genome editing），重现了两类此类毒素基因的演化历程——细胞致死膨胀毒素B（cytolethal distending toxin B，cdtB）与56kDa凋亡诱导蛋白（apoptosis inducing protein of 56kDa，aip56）。这两类基因很可能在数百万年前从内共生细菌的噬菌体中水平转移至昆虫基因组。研究发现，在安娜果蝇亚群（D. ananassae subgroup）物种中保守存在的cdtB::aip56融合基因（fusionB），当在黑腹果蝇的免疫组织中异源表达时，可显著提升果蝇存活率，并抑制寄生蜂的发育。研究发现……，以及

# 实验性将噬菌体来源基因水平转移至果蝇（*Drosophila*）中可赋予宿主对寄生蜂的先天免疫力

https://doi.org/10.5061/dryad.q573n5tsh

## 数据与文件结构说明

本仓库包含论文《实验性将噬菌体来源基因水平转移至果蝇（*Drosophila*）中可赋予宿主对寄生蜂的先天免疫力》中所述基因组分析与计数类实验的原始数据。

作者：Rebecca L. Tarnopol*、Josephine Tamsil、Gyöngyi Cinege、Ji Heon Ha、Kirsten I. Verster、Edit Ábrahám、Lilla B. Magyar、Bernard Y. Kim、Susan L. Bernstein、Zoltán Lipinszki、István Andó、Noah K. Whiteman

请引用相关论文：https://doi.org/10.1016/j.cub.2024.11.071

### 文件与变量

#### 文件：Fig1_rawdata.zip

**描述：** 包含图1及相关补充图生成所需的原始数据

> ananassae_final_wh...