Comparison of gene expression signatures between Cre- KTC organoid organoid and Cre+ KTC organoid

To identify downstream genes involved in cholangiocarcinoma development by genetic manipulations of Kras, TGFbR2, and CDH1, we cultured the biliary organoid from the extrahepatic bile duct of Cre-negative LSL-KrasG12D;Tgfbr2flox/flox;CDH1flox/flox (KTC) mice, and induced gene recombination using a lentivirus expressing Cre-recombinase (Cre+ KTC organoid) or control lentivirus (Cre- KTC organoid). Then, we compared gene expression signatures between Cre- KTC organoid organoid and Cre+ KTC organoid. Biliary organoids isolated from KTC mice were infected with Cre-expressing or control lentivirus, and transduced cells were selected using puromycin. Two weeks later, we compared gene expression signatures between Cre- KTC organoid organoid and Cre+ KTC organoid.