Investigating the association between diet and infection with <em>Trichomonas gallinae</em> in the European turtle dove (<em>Streptopelia turtur</em>)
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Trichomonas gallinae, a protozoan parasite infecting a wide range of birds, has been a cause for conservation concern since its recognition as an emerging infectious disease, having had notable impacts on several avian species, including causing widespread mortality in greenfinches (Chloris chloris), and chaffinches (Fringilla coelebs), and hindering the recovery of the endangered pink pigeon (Nesoenas mayeri). Horizontal transmission between birds congregating at feeding sites, such as those provided for conservation and species management purposes, is an important driver in the spread of T. galliane. Supplementary feeding is a key conservation intervention for the European turtle dove (Streptopelia turtur), which is declining across its range, driven at least partially by a loss of natural food resources. Due to the link between T. gallinae transmission and supplementary feeding, we consider the prevalence of this parasite amongst European turtle doves in relation to diet in the first..., Faecal samples and buccal cavity swab samples were collected from wild-caught European Turtle Doves (Streptopelia turtur). DNA was extracted from these samples, amplified by PCR, then sequenced using High-Throughput Sequencing. Bioinformatic data processing was completed to identify dietary items consumed by birds sampled (faecal samples) and the status of infection with the protozoan parasite Trichomonas galliane (buccal swab samples). Statistical analysis was carried out using R. , , # Investigating the association between diet and infection with Trichomonas gallinae in the European turtle dove (Streptopelia turtur) [https://doi.org/10.5061/dryad.8kprr4xt1](https://doi.org/10.5061/dryad.8kprr4xt1) ## Description of the data and file structure These data sets contain both dietary and parasite infection data collected from European turtle dove (*Streptopelia turtur*) on both the breeding and wintering grounds. Data sets used in analysis are post-bioinformatic processing. Dietary data is obtained from faecal samples, using high throughput sequencing. Data on *Trichomonas galliane* infection and strains were obtained from DNA isolated from swabs of the oral cavity, oesophagus, and crop. High-throughput sequencing was used to detect strains present in samples. The results of this study look at infection with *T. gallinae* in relation to diet. ### Files and variables #### File: 1._Detrended_correspondence_analysis_and_GLM_including_DCA_variables_script.R **Descript...,
禽毛滴虫（*Trichomonas gallinae*）是一种广泛寄生于多种鸟类的原生动物寄生虫，自被认定为新发传染病以来，便一直受到保护生物学领域的高度关注。该寄生虫对多种鸟类造成了显著危害：导致红额金翅雀（*Chloris chloris*）、苍头燕雀（*Fringilla coelebs*）出现大规模死亡，同时阻碍了濒危物种粉鸽（*Nesoenas mayeri*）的种群恢复。 鸟类在取食场所（如为保护及物种管理目的设立的投喂点）聚集时发生的水平传播，是禽毛滴虫传播的重要驱动因素。人工投喂是欧斑鸠（*Streptopelia turtur*）的一项核心保护干预措施。该物种的种群在全球分布范围内持续下降，其原因至少部分源于天然食物资源的减少。鉴于禽毛滴虫传播与人工投喂之间的关联，本研究首先针对欧斑鸠体内该寄生虫的感染率与饮食的相关性展开研究。研究人员从野外捕获的欧斑鸠（*Streptopelia turtur*）体内采集了粪便样本与口腔拭子样本。从上述样本中提取DNA，通过聚合酶链式反应（Polymerase Chain Reaction，PCR）进行扩增，随后采用高通量测序（High-Throughput Sequencing）进行测序。完成生物信息学数据处理后，研究人员分别通过粪便样本鉴定受试鸟类的摄食食物种类，通过口腔拭子样本检测其感染禽毛滴虫的情况。统计分析采用R语言完成。 # 欧斑鸠（*Streptopelia turtur*）饮食与禽毛滴虫感染相关性研究 https://doi.org/10.5061/dryad.8kprr4xt1 ## 数据与文件结构说明 本数据集包含从繁殖地与越冬地采集的欧斑鸠（*Streptopelia turtur*）的饮食数据与寄生虫感染数据。用于分析的数据集均经过生物信息学后处理。饮食数据通过高通量测序分析粪便样本获得。禽毛滴虫感染情况及虫株信息则通过从口腔、食道和嗉囊拭子中提取的DNA进行检测，同时采用高通量测序鉴定样本中的虫株类型。 本研究结果聚焦于禽毛滴虫感染与饮食的相关性分析。 ### 文件与变量说明 #### 文件：1._Detrended_correspondence_analysis_and_GLM_including_DCA_variables_script.R **描述……**
