Temporal properties of mouse hippocampal CA3 area PV and CCK inhibitory neuron transmission measured by physiologically relevant action potential sequences.

The data set contains raw data and values of inhibitory transmission parameters
(IPSC rise, decay, amplitude and short-term plasticity) between interneurons and
their interneuron/pyramidal cell targets in response to for different physiologically
relevant firing patterns. We measured the parameters of transmission from pairs of
CA3 area PV positive basket and axo-axonic, as well as CCK positive basket and dendrite
targeting neurons in mouse (postnatal 19-25 days) in vitro hippocampal slices.
Presynaptic cells were stimulated by a train of 10 action potentials at frequencies
physiological in the hippocampus (1 Hz, 5 Hz, 10 Hz, 20 Hz, 40 Hz, 80 Hz, 160 Hz, 320 Hz).
We also tested the response of connections to inputs reflecting the behavior of
perisomatic-region targeting interneurons during sharp wave-ripples (RIP protocol
- 4 APs at 160 Hz) and pathological interictal events (EPI protocol - 30 APs at 160 Hz
followed by 4 APs at 320 Hz). The time constant of recovery from inhibition was calculated
using stimuli, which consisted of two consecutive RIP protocols where the time between
the two RIP patterns was systematically varied. The intervals used were 50 ms, 100 ms,
200 ms, 300 ms, 500 ms, 800 ms, and 1200 ms. We present the raw experimental data as
well as data tables characterizing (amplitude, success rate, etc.) the transmission
for all recorded cell pairs. A summary table helps orientation.

本数据集包含中间神经元与其中间神经元/锥体神经元靶细胞之间的抑制性传递参数原始数据及对应数值，具体包括抑制性突触后电流（IPSC, inhibitory postsynaptic current）的上升时程、衰减时程、峰值幅度以及短时程可塑性参数，上述参数为不同生理相关放电模式下的响应记录数据。我们通过成对神经元记录，测量了出生后19-25天的小鼠体外海马脑片中，CA3区小清蛋白（PV, parvalbumin）阳性篮状细胞、轴突-轴突细胞（axo-axonic cell），以及胆囊收缩素（CCK, cholecystokinin）阳性篮状细胞和树突靶向神经元（dendrite-targeting neuron）的突触传递参数。突触前神经元被施加10个动作电位的串刺激，刺激频率覆盖海马脑区的生理范围：1 Hz、5 Hz、10 Hz、20 Hz、40 Hz、80 Hz、160 Hz、320 Hz。此外，我们还测试了两种特殊输入模式下的突触连接响应：其一对应尖波涟漪（sharp wave-ripples, SWR）期间胞体周围区域靶向中间神经元的活动（RIP方案：以160 Hz频率施加4个动作电位）；其二对应病理性发作间期事件（interictal events）（EPI方案：以160 Hz频率施加30个动作电位，随后以320 Hz频率施加4个动作电位）。我们通过连续两次RIP方案刺激、且两次RIP模式间间隔系统变化的实验范式，计算了抑制作用的恢复时间常数，所用间隔时长为50 ms、100 ms、200 ms、300 ms、500 ms、800 ms及1200 ms。本数据集既提供了原始实验数据，也包含所有记录细胞对的突触传递特性数据表（涵盖幅度、成功率等参数），同时附带一张汇总表以方便快速查阅。