anti-NONO RIP seq in liver nuclei after fasting and re-feeding

In order to identify the RNAs bound by NONO complexes in different nutritional conditions, we carried out RNA immunoprecipitation followed by sequencing (RIP-seq) of NONO from mouse liver nuclei collected at three different times of the day. Samples were collected after fasting (ZT10), 2h after re-feeding (ZT14) and towards the end of the feeding period (ZT22). The co-immunoprecipitated RNA (along with input RNA and unspecific IgG bound RNA) was sequenced. Livers were collected at 3 different time points. RIP and Input RNA were sequenced for each time point and one IgG control for a total of 8 samples. Each sample is a pool of 2 independent experiments.